Adenovirus vector is one of the most widely used viral vectors for gene therapy. Compared with other viral vectors, adenovirus has various advantages such as high transduction effi ciency, broad tropism for diff erent tissues and epichromosomal persistence in the host cell, etc. Adenovirus vectors can be modifi ed as replication-deficient and replication-selective oncolytic virus vector. The strong immunogenicity of adenovirus vector makes it suitable to be applied in genetic vaccines and anti-cancer therapies. At present, many clinical trials have confirmed the safety and effectiveness of adenovirus vector in the above-mentioned therapeutic attempts.